Pharmacokinetics Analysis
Pharmacokinetics (PK) analysis is a core component of modern drug discovery, evaluating absorption, distribution, metabolism, and excretion (ADME) properties of lead compounds to predict in vivo performance, optimize molecular structure, reduce clinical failure risk, and support lead-to-candidate progression. As a trusted brand under STEMart, PharmaAnalytica delivers standardized, high-throughput, and regulatory-oriented PK services for global pharmaceutical and biotech clients. Our platform integrates advanced in vitro permeability models, quantitative bioanalysis, and high‑resolution mass spectrometry to provide full-cycle ADME characterization for lead compounds.
Specialized Pharmacokinetics Analysis Services
Parallel Artificial Membrane Permeability Assay (PAMPA)
PAMPA is a high-throughput in vitro model for evaluating passive transcellular permeability of lead compounds, widely used for early absorption prediction and rapid screening. It simulates lipid membrane barriers without cell involvement, enabling fast, stable, and repeatable ranking of oral absorption potential. This assay supports large-scale compound optimization and is ideal for early-stage lead filtering.
Caco-2 Permeability Assay
Caco-2 cell monolayer is the gold‑standard in vitro model for predicting human intestinal absorption and transmembrane transport. It distinguishes passive diffusion, active uptake, and efflux transport (e.g., P‑gp), supporting assessment of oral bioavailability, drug-drug interaction risk, and formulation effects. Reliable Caco-2 data directly guides structural modification and candidate selection.
Drug Distribution and Excretion Analysis with LC‑MS/MS
Quantitative analysis of drug concentration in plasma, tissue, bile, urine, and feces is performed using validated LC-MS/MS systems to obtain key PK parameters: AUC, Cmax, Tmax, t1/2, Vd, CL, and cumulative excretion. This service reveals in vivo distribution characteristics and elimination pathways, supporting dose design, tissue accumulation assessment, and safety evaluation.
Metabolite Identification with Q‑TOF MS
High-resolution quadrupole time-of-flight mass spectrometry (Q-TOF MS) is used for unbiased identification and structural characterization of drug metabolites in plasma, bile, and liver microsomal incubations. It enables detection of phase I and phase II metabolites, clarification of metabolic pathways, and identification of reactive intermediates, supporting safety assessment and metabolic soft-spot optimization.
High-Performance Analytical Technology & Instrument Platform
LC MS/MS Quantitative Bioanalysis System
LC-MS/MS provides high sensitivity, wide linear range, and excellent selectivity for precise quantification of lead compounds and metabolites in complex biological matrices. The system supports multi‑reaction monitoring (MRM) mode, delivering high-throughput detection with ppt-level sensitivity.
Q TOF High Resolution Mass Spectrometry System
Q-TOF MS enables high‑accuracy full-scan detection and elemental composition analysis for unknown metabolite identification. It supports untargeted metabolite profiling, MS/MS structural elucidation, and isotopic distribution matching, allowing efficient detection and structural confirmation of oxidative, hydrolytic, and conjugated metabolites.
Automated Liquid Handling & Sample Preparation Platform
Integrated automated extraction, protein precipitation, solid‑phase extraction (SPE), and derivatization modules ensure standardized, reproducible sample processing. High‑throughput workflows reduce manual error and improve data reliability for large-scale PK studies.
Why Choose PharmaAnalytica?
Full ADME-PK Technology Ecosystem
Our integrated platform covers permeability screening, quantitative bioanalysis, tissue distribution, excretion profiling, and metabolite identification, providing one‑stop pharmacokinetics evaluation for lead compounds.
Advanced Mass Spectrometry & Chromatography Instruments
High-sensitivity LC-MS/MS and high-resolution Q-TOF MS systems ensure accurate quantification and reliable structural identification, supporting early drug discovery and lead optimization.
Standardized & GLP-Oriented Management
All methods are validated with specificity, linearity, accuracy, precision, recovery, and stability. Complete data traceability, audit trails, and standardized reports support preclinical development and regulatory submissions.
Professional PK Expert Team
Our scientists provide in-depth interpretation of absorption, distribution, metabolism, and excretion characteristics, and deliver actionable guidance for molecular optimization, candidate selection, and development progression.
Pharmacokinetics analysis is essential for derisking lead compounds and accelerating clinical translation. Backed by STEMart's quality system, PharmaAnalytica combines advanced instrumentation, standardized methods, andprofessional expertise to support efficient, reliable, and innovation-driven drug discovery.
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